The Effects of Nickel Oxide Nanoparticles on Structural Changes, Heme Degradation, Aggregation of Hemoglobin and Expression of Apoptotic Genes in Lymphocyte Cells

Salihi, Abbas (2019) The Effects of Nickel Oxide Nanoparticles on Structural Changes, Heme Degradation, Aggregation of Hemoglobin and Expression of Apoptotic Genes in Lymphocyte Cells. Journal of Biomolecular Structure and Dynamics, 38 (12). pp. 3676-3686. ISSN 07391102

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Abstract

Nickel oxide nanoparticles (NiO NPs) have received great interests in medical and biotechnological applications. However, their adverse impacts against biological systems have not been well-explored. Herein, the influence of NiO NPs on structural changes, heme degradation and aggregation of hemoglobin (Hb) was evaluated by UV-visible (Vis), circular dichroism (CD), fluorescence, transmission electron microscopy (TEM), and molecular modeling investigations. Also, the morphological changes and expression of Bax/Bcl-2 mRNA in human lymphocyte cell exposed to NiO NPs were assayed by DAPI staining and quantitative real-time PCR (qPCR), respectively. The UV-Vis study depicted that NiO NPs resulted in the displacement of aromatic residues and heme groups and production of the pro-aggregatory species. Intrinsic and Thioflavin T (ThT) fluorescence studies revealed that NiO NPs resulted in heme degradation and amorphous aggregation of Hb, respectively, which the latter result was also confirmed by TEM study. Moreover, far UVCD study depicted that NiO NPs lead to substantial secondary alteration changes of Hb. Furthermore, near UV-CD displayed that NiO NPs cause quaternary structural changes of Hb as well as heme displacement. Molecular modelling study also approved that NiO NPs resulted in structural changes of Hb and heme deformation. Moreover, morphological and genotoxicity assays revealed that the DNA fragmentation and expression ratio of Bax/Bcl-2 mRNA increased in lymphocyte cells treated with NiO NPs for 24 hr. In conclusion, this study indicates that NiO NPs may affect the biological media and their applications should be limited

Item Type: Article
Uncontrolled Keywords: Interaction, Nickel oxide, Nanoparticles, Hemoglobin, Heme degradation, Aggregation, Genotoxicity
Subjects: Q Science > QM Human anatomy
Q Science > QR Microbiology
R Medicine > R Medicine (General)
Depositing User: ePrints deposit
Date Deposited: 04 Jan 2021 06:07
Last Modified: 04 Jan 2021 06:07
URI: http://eprints.tiu.edu.iq/id/eprint/299

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