Phytochemical analysis and evaluation of antibacterial activity of Moringa oleifera extracts against gram-negative bacteria: an in vitro and molecular docking studies

R. Ganjo, Aryan and N. Adham, Aveen and A. Al-Bustany, Hazem and T. Aka, Safaa (2023) Phytochemical analysis and evaluation of antibacterial activity of Moringa oleifera extracts against gram-negative bacteria: an in vitro and molecular docking studies. Current Issues in Pharmacy and Medical Sciences, 35 (4). pp. 198-205.

Text (Research Article) 10.2478_cipms-2022-0035.pdf - Published Version Download (2MB)

Abstract

Moringa oleifera seed and leaf are used traditionally for the treatment of various healthproblems (among others, hypertension, scrapes, skin infection, diabetes, genitourinaryillnesses), and to boost the immune system, as well as to act as a contraceptive. Inthis study, the antibacterial activity of seed and leaf M. oleifera extracts on three-gramnegative bacteria was investigated, and phytochemical analysis for the association ofantibacterial activity with the active constituents in the plant was determined. Moreover,understanding of the mechanism of action was achieved by applying the Auto DockVina technique. The phytochemical screening of M. oleifera seed and leaf extractsexhibited the presence of alkaloids, carbohydrates, cardioactive glycosides, flavonoids,tannins, phenols, steroids and terpenoids. In silico results revealed that compounds(4-O-caffeoyl quinic acid, 4-(α-L-rhamnopyranosyloxyl)-benzylisothiocyanate);(Isoquercitrin, 4-(α-L-rhamnopyranosyloxy) benzyl glucosinolate); and (Astragalin,4-(α-L-rhamnopyranosyloxy) benzyl glucosinolate) from leaf and seed have the highestbinding affinity and very good interactions with Transcriptional Activator Protein (LasR),Klebsiella pneumoniae carbapenemase (KPC), and Malonyl-CoA-acyl carrier proteintransacylase (FabD), respectively.

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