Comparing The Antibacterial Efficacy of NaoCL, Chlorhexidine, NaOCL/MTAD Chlorhexidine/MTAD Against E. Faecalis

A. Thiab, Karam and Salim Younus, Mohamed (2023) Comparing The Antibacterial Efficacy of NaoCL, Chlorhexidine, NaOCL/MTAD Chlorhexidine/MTAD Against E. Faecalis. Eurasian Journal of Science and Engineering, 9 (1).

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Abstract

Aim of study: This study aims at comparing the anti-microbial efficacy of irrigation with Biopure MTAD against irrigation with 5.25 % NaOCl, CHX, NaOCl/MTAD, and CHX/MTAD in E. faecalis-root canals. Materials and Methods: Thirty (30) human single-rooted lower premolar teeth were extracted and de-coronated at the cemento-enamel junction (CEJ). Then, the roots were instrumented by k-file until the size is 40. Furthermore, these teeth were exposed to bacterial contamination in brain heart infusion (BHI) for 48 h. The contaminated samples were divided into 5 groups (MTAD, NaOCl, CHX, NaOCl / MTAD, and CHX / MTAD). The dentin chips in the canals of the samples were removed using a sterile low-speed handpiece round bur and transferred to BHI. The media was cultured, and the bacterial colonies were counted and recorded as CFU/mL. Results: NaOCl / MTAD showed a significantly higher antimicrobial activity compared to 5.25 % NaOCl, MTAD, CHX, and CHX / MTAD. Conclusion: The antimicrobial activity of a combined NaOCl and Biopure MTAD was strongest against E. faecalis compared to the other irrigant solutions.

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