Curcumin and Berberine Arrest Maturation and Activation of Dendritic CellsDerived from Lupus Erythematosus Patients

Reza Nikpoor, Amin and Mahmoudi, Mahmoud and Shapouri-Moghaddam, Abbas and Rezaieyazdi, Zahra and Mollazadeh, Samaneh and Tabasi, Nafiseh and Mansouri, Atena and Modarres Moghadam, Reyhane and Abbas Momtazi, Amir and Kay Fi Najmaldin, Soran and Kamal Kheder, Ramiar and Esmaeili, Seyed-Alireza (2024) Curcumin and Berberine Arrest Maturation and Activation of Dendritic CellsDerived from Lupus Erythematosus Patients. Current Molecular Pharmacology, 17. ISSN 1874-4702

Text (Research Article) CMP-17-e18761429249908.pdf - Published Version Download (9MB)

Abstract

Background: Systemic lupus erythematosus (SLE) is a complex autoimmune disease recognized by elevated activity of autoimmune cells, loss of tolerance, and decreased regulatory T cells producing inhibitory cytokines. Despite many efforts, the definitive treatment for lupus has not been fully understood. Curcumin (CUR) and berberine (BBR) have significant immunomodulatory roles and anti-inflammatory properties that have been demonstrated in various studies. This study aimed to investigate the anti-inflammatory properties of CUR and BBR on human monocyte-derived dendritic cells (DCs) with an special focus on the maturation and activation of DCs. Methods: Human monocytes were isolated from the heparinized blood of SLE patients and healthy individuals, which were then exposed to cytokines (IL-4 and GM-CSF) for five days to produce immature DCs. Then, the obtained DCs were characterized by FITC-uptake assay and then cultured in the presence of CUR, BBR, or lipopolysaccharide (LPS) for 48 h. Finally, the maturation of DCs was analyzed by the level of maturation using flow cytometry or real-time PCR methods. Results: The results showed promising anti-inflammatory effects of CUR and BBR in comparison with LPS, supported by a significant reduction of not only co-stimulatory and antigen-presenting factors such as CD80, CD86, CD83, CD1a, CD14, and HLA-DR but also inflammatory cytokines such as IL-12. Conclusion: CUR and BBR could arrest DC maturation and develop a tolerogenic DC phenotype that subsequently promoted the expression of inhibitory cytokines and reduced the secretion of proinflammatory markers.

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