Effect of Different Levels of Hesperetin on the Quality of Rooster Semen

Abdalkarim Salih, Sahar and Abd Hussein, Rozhgar and Majeed Noori, Imad and Hussein Bapir, Shorsh and Maulud Hamad, Kareem and Wasman Hamad, Saber (2023) Effect of Different Levels of Hesperetin on the Quality of Rooster Semen. Passer Journal of Basic and Applied Sciences, 5 (2). pp. 417-421. ISSN 27065952

Text (Research Article) PSR_Volume 5_Issue 2_Pages 417-421.pdf - Published Version Download (220kB)

Abstract

The study was examined the impact of varying concentrations of hesperetin on the cryopreservation of rooster sperm. Sperm were diluted with a Lake Extender containing (0, 15, 20, 25, and 30) μM hesperetin. After a 30-second freeze-thaw operation at 37 °C, membrane integrity, sperm motility characteristics, aberrant morphology, apoptotic status, and mitochondrial activity were assessed. The concentration of 25 μM hesperetin observed the highest values (P < 0.01) were (64.26 %, 30.44 %, 35.88 mm/s, 21.77 mm/s, 61.08 mm/s, 35.66%, 61.14%, 16.23 Hz, 61.71%, 61.71%, and 61.33%) for (Total motility, progressive motility, average velocity path, curved velocity, straight-line velocity, linearity, straightness, beat cross frequency, membrane integrity, mitochondrial activity, and viability, respectively). In contrast, it had the lowest values (3.94mm, 13.67%, 13.30%, and 25.37%) recorded for lateral head displacement amplitude, total abnormality, apoptotic sperm, and dead sperm, respectively. It may be concluded that adding 25 μM hesperetin is an effective method for preserving the quality of cryopreserved rooster sperm.

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