Association study of Interleukin 10 gene polymorphisms in Iraqi patients with multiple sclerosis

Wasman Smail, Shukur and Omar Khudhur, Zhikal (2020) Association study of Interleukin 10 gene polymorphisms in Iraqi patients with multiple sclerosis. Gene Reports. ISSN 24520144

Text (Research Article) qadir2020 (1).pdf - Published Version Download (501kB)

Abstract

Multiple sclerosis (MS) is a type of autoimmune disease where immune cell attacks our cells mistakenly; its severity is measured by expanded disease status scale (EDSS). The study aims the investigation of −1082 polymorphism in interleukin 10 (IL-10) as one of the etiologies that develops the disease. This is a case-control study that allele-specific polymerase chain reaction (AS-PCR) were provided to compare 100 relapsing-remitting MS (RRMS) patients, which fulfills McDonald criteria with 100 healthy controls depending on the −1082 (G/A) polymorphism of the gene encoding IL-10. The A allele frequency of IL-10 gene has been considerably less in MS patients compare to healthy control (60.50 Vs. 81%). Genotype distributions of the single nucleotide polymorphism (SNP) -1082 fulfills Hardy-Weinberg equilibrium in cases (P = 0.155) but it doesn't in controls (P < 0.0001). In MS patients, Heterozygous (GA) genotypes were non-significantly associated with MS (OR = 0.834,95% CI = 0.6890 to 1.29, P 0.706) but homozygous (AA) were significantly associated with this condition (OR = 3.420, 95% CI = 1.450 to 8.065, P = 0.0037). To conclude, the genotype distribution of −1082 (G/A) polymorphism has been showed a significant difference in the case/control study recruited in Erbil province-Iraq, and EDSS is significantly higher in A allele's carrier genotypes. There was non-significance association AA genotypes and duration of the disease.

Actions (login required)

View Item